Determination of Transgene Copy Number and Expression Level Using Denaturing Gradient Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Determination of transgene copy number and expression level using denaturing gradient gel electrophoresis.
Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models. Currently, transgene copy number and expression are estimated by Southern blot analysis of genomic DNA and Northern blot analysis of mRNA. We report a novel PCR-based method...
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and -IgM k and l light chains did not show the presence of monoclonal components. Reduction with mercaptoethanol (1:2 dilution), which dissociates complexes but does not dissociate albumin dimers, excluded the possible formation of complexes with a1-antitrypsin. Interferences by radio-opaque agents or medications, which could lead to the appearance in the CZE electropherograms of abnormal peaks...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98241st06